rpmE [2019-07-08 10:49:27]

ribosomal protein

locus: BSU37070

pI: 9.16

mw: 7.31 kDa

protein length: 66 aa

gene length: 201 bp

function: translation

product: ribosomal protein L31

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 3,803,081 3,803,281

Phenotypes of a mutant

• delayed spore [SW|germination] [Pubmed|25661487]

The protein

Protein family

• Type A subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|RpmEB]

[SW|Cofactors]

• requires zinc for activity [Pubmed|19648245,15049826]

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

Additional information

• Under conditions of zinc starvation, RpmE is replaced by its paralog [protein|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|RpmEB], that does not require zinc [Pubmed|15049826]

Expression and Regulation

Operons

• genes: [gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
  description: [Pubmed|9353933]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|035154A4160F084BA1CC124D0951937B203E167D|RpmE]: autorepression, [pubmed|32448158], in [regulon|035154A4160F084BA1CC124D0951937B203E167D|RpmE regulon]

  regulation

  • autorepression of [gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE] expression upon binding of excess [protein|035154A4160F084BA1CC124D0951937B203E167D|L31] to a [SW|RNA switch] in the untranslated region of the [gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE] mRNA [Pubmed|32448158]
  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

• genes: [gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE37070 ([gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA
• BKK37070 ([gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTATCCATCTCCTTCC, downstream forward: _UP4_TAATAATAGATTTCTCAACA

References

• 17163968,15049826,19648245,19653700,20208344,23002217,25661487,25903689